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目的探讨癌基因HER-2反义寡核苷酸联合放疗对乳腺癌细胞的影响。方法实验分组如下:HER-2反义寡脱氧核苷酸组、HER-2正义寡脱氧核苷酸组、叶酸对照组和空白对照组。用RT-PCR方法、MTT法及平板克隆形成实验检测乳腺癌细胞SK-Br-3受药物处理后,经60Coγ射线照射,HER-2表达水平、细胞存活分数和集落形成率的改变。结果经HER-2反义寡核苷酸-叶酸处理的细胞,对60Coγ射线的放射敏感性增加,细胞的HER-2癌基因表达水平降低,存活分数及集落形成率与正义寡核苷酸、叶酸和空白对照组比较明显降低(P<0.05),而正义寡核苷酸、叶酸与空白对照组相比无明显差异(P>0.05)。结论HER-2反义寡核苷酸抑制癌基因表达的作用增加乳腺癌细胞SK-Br-3对放疗的敏感性。
Objective To investigate the effect of oncogene HER-2 antisense oligonucleotide combined with radiotherapy on breast cancer cells. Methods The experimental groups were as follows: HER-2 antisense oligodeoxynucleotide group, HER-2 sense oligodeoxynucleotide group, folic acid control group and blank control group. The expression of HER-2, cell viability and colony formation in SK-Br-3 breast cancer cells treated with 60Coγ ray were detected by RT-PCR, MTT assay and plate clone formation assay. Results The HER-2 antisense oligodeoxynucleotide-folic acid-treated cells showed increased radiosensitivity to 60Coγ-rays, decreased HER-2 oncogene expression, survival and colonization rates, Folic acid and blank control group was significantly lower (P <0.05), while the sense oligonucleotide and folic acid compared with the blank control group had no significant difference (P> 0.05). Conclusion The effect of HER-2 antisense oligonucleotide on oncogene expression increases the sensitivity of breast cancer cell line SK-Br-3 to radiotherapy.