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第3代基因组定点编辑技术(Clustered Regularly Interspaced Short Palindromic Repeat(CRISPR,成簇规律间隔短回文序列))介导的Cas9核酸酶系统,已被用于真核细胞的基因组编辑和基因修饰。CRISPR/Cas9技术有许多潜在的应用,包括对人类基因组进行编辑以治疗遗传缺陷疾病或基因突变疾病;删除病原体基因(如细菌基因组或病毒基因组),消灭病原体,达到消除传染性疾病的目的;也可用于农业生产(包括植物和动物的基因)中农作物的改造。综述基因组定点编辑技术CRISPR/Cas9在病毒感染性疾病和遗传缺陷性疾病中的应用文献,并对其研究进展作了分析。
The Cas9 nuclease system mediated by the third generation of Genome-wide Site-directed Editing (CRISPR) sequences has been used for genomic editing and genetic modification of eukaryotic cells. There are many potential applications of CRISPR / Cas9 technology, including editing of the human genome to treat genetic defects or mutations; deleting pathogens (such as bacterial genomes or viral genomes) and eliminating pathogens to eliminate infectious diseases; and Can be used for agricultural production (including plants and animal genes) in the transformation of crops. This review summarizes the applications of genome-targeted editing technology CRISPR / Cas9 in viral infectious diseases and genetic defects, and analyzes its progress.