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目的 观察转化生长因子 β1(TGFβ1)及其Ⅱ型受体 (TGFβRⅡ )在宫颈鳞状上皮癌中的表达情况 ,探讨其与宫颈癌发生发展的关系。方法 应用免疫组化SP法检测 4 0例宫颈鳞状上皮癌 (病理分级Ⅰ -Ⅲ级 )中TGFβ1和TGFβRⅡ的蛋白表达 ,并与正常宫颈组织及慢性宫颈炎组织作半定量比较。结果 (1)正常宫颈组织及慢性宫颈炎组织中TGFβ1和TGFβRⅡ表达于上皮细胞的胞浆内 ,两组表达水平差异无显著性 (P >0 .0 5 ) ;宫颈鳞状上皮癌组织中TGFβ1和TGFβRⅡ蛋白表达位于癌细胞的胞浆内 ;(2 )宫颈鳞状上皮癌组织中TGFβ1蛋白表达水平明显上升 ,与前二组比较差异有显著性 (P <0 .0 1) ;(3 )宫颈鳞状上皮癌组织中TGFβRⅡ蛋白表达明显下降 ,与前二组比较差异有显著性 (P <0 .0 1) ;(4)随着宫颈癌病理分级的增加 ,TGFβ1表达水平上升 ,其差异也有显著性 (P <0 .0 5 ) ,但TGFβRⅡ表达无显著性差异。结论 宫颈鳞状上皮癌的发生发展可能与TGFβ1蛋白的高表达和TGFβRⅡ蛋白的低表达有关。
Objective To observe the expression of transforming growth factor β1 (TGFβ1) and its type Ⅱ receptor (TGFβR Ⅱ) in cervical squamous cell carcinoma and to explore its relationship with the occurrence and development of cervical cancer. Methods Immunohistochemical SP method was used to detect the protein expression of TGFβ1 and TGFβRⅡ in 40 cases of cervical squamous cell carcinoma (pathological grade Ⅰ-Ⅲ grade), and compared with normal cervical tissue and chronic cervicitis. Results (1) The expressions of TGFβ1 and TGFβRⅡ in normal cervical tissue and chronic cervicitis were significantly higher in the cytoplasm of epithelial cells than that in normal cervix and chronic cervicitis (P> 0.05) (2) The expression of TGFβ1 protein in cervical squamous cell carcinoma tissue was significantly increased (P <0.01); (3) The expression of TGFβRⅡprotein in cervical squamous cell carcinoma was significantly higher than that in the first two groups The expression of TGFβRⅡprotein in cervical squamous cell carcinoma was significantly lower than that in the first two groups (P <0.01). (4) With the increase of pathological grade of cervical cancer, the expression level of TGFβ1 increased There was also significant (P <0.05), but there was no significant difference in the expression of TGFβRⅡ. Conclusion The occurrence and development of cervical squamous cell carcinoma may be related to the high expression of TGFβ1 protein and the low expression of TGFβRⅡ protein.