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目的该研究探讨了高压氧(HBO)对缺氧缺血性脑损伤(HIBD)新生大鼠内源性神经干细胞(NSCs)的迁移与分化的影响。方法7日龄Sprague-Dawley新生大鼠随机分为对照组,模型组及HBO组。采用Rice-Vannucci方法制成HIBD模型。造模后3 h内行HBO治疗。分别于HBO治疗后7 d、14 d、28 d,采用BrdU/DCX,BrdU/β-tubulin,BrdU/GFAP和BrdU/O4免疫荧光双标法,用共聚焦显微镜动态检测侧脑室室管膜下区(sub-ventricular zone,SVZ)与大脑皮层内源性NSCs的迁移与分化。结果治疗后7 d,HBO组损伤侧SVZ区BrdU+DCX+细胞数(84±21个/mm2)增加,多于对照组(39±14个/mm2)与模型组(68±17个/mm2)(P<0.05);治疗后14 d,SVZ区BrdU+DCX+细胞数减少,而皮层区BrdU+DCX+细胞数增加,HBO组明显多于对照组(P<0.01);治疗后28 d,各组大脑皮层BrdU+DCX+细胞数减少,大脑皮层出现BrdU+β-tubulin+,BrdU+GFAP+,BrdU+O4+细胞。HBO组BrdU+β-tubulin+与BrdU+O4+细胞数显著高于对照组与模型组(P<0.05)。结论高压氧可促进HIBD新生大鼠内源性NSCs迁移到大脑皮层并分化为成熟的神经细胞。
Objective To investigate the effects of hyperbaric oxygen (HBO) on the migration and differentiation of endogenous neural stem cells (NSCs) in neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods 7-day-old Sprague-Dawley newborn rats were randomly divided into control group, model group and HBO group. HIBD model was made by Rice-Vannucci method. HBO treatment within 3 h after modeling. BrdU / DCX, BrdU / β-tubulin, BrdU / GFAP and BrdU / O4 double immunofluorescence double staining were used to detect the subventricular zone of the lateral ventricle at 7d, 14d and 28d after HBO treatment respectively Migration and differentiation of sub-ventricular zone (SVZ) and endogenous NSCs in cerebral cortex. Results After 7 days of treatment, the number of BrdU + DCX + cells in the injured area of HBO group increased (84 ± 21 cells / mm2) more than that in the control group (39 ± 14 cells / mm2) and the model group (68 ± 17 cells / mm2) (P <0.05). On the 14th day after treatment, the number of BrdU + DCX + cells in SVZ decreased, while the number of BrdU + DCX + cells in cortex increased, while that in HBO group was significantly higher than that in control group BrdU + DCX + cells decreased in the cerebral cortex, BrdU + β-tubulin +, BrdU + GFAP + and BrdU + O4 + cells in the cerebral cortex. The number of BrdU + β-tubulin + and BrdU + O4 + cells in HBO group was significantly higher than that in control group and model group (P <0.05). Conclusion Hyperbaric oxygen can promote the migration of endogenous NSCs into the cerebral cortex and differentiate into mature neural cells in HIBD neonatal rats.