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目的建立用HPLC法测定心脑联通分散片中野黄芩苷的含量。方法采用HPLC法,Kromasil C18色谱柱(5μm,4.6×250 mm);柱温:40℃;流动相为甲醇-0.1%磷酸溶液(40:60);检测波长335 nm。以野黄芩苷为对照品,外标法定量。结果野黄芩苷在21.60～108.00 ug/mL范围内线性关系良好(r=0.9999),平均加样回收率为98.25%,RSD=1.04%。结论本法操作简便、准确,重现性好,可用于该制剂的质量控制。
Objective To establish a method for the determination of wild baicalin in Xinnao Lian Tong Dispersible Tablets by HPLC. Methods The HPLC method was applied on a Kromasil C18 column (5μm, 4.6 × 250 mm). The column temperature was 40 ℃. The mobile phase was methanol-0.1% phosphoric acid solution (40:60). The detection wavelength was 335 nm. To baicalin as a reference substance, external standard method. Results Scutellaria baicalensis had a good linearity (r = 0.9999) in the range of 21.60-108..00 ug / mL with an average recovery of 98.25% and a RSD of 1.04%. Conclusion This method is simple, accurate, reproducible and can be used for the quality control of the preparation.