The construction of flexible platform possessing the functions of immobilizing, separating, rinsing, and high-throughput analysis plays a significant role in biological and clinical research. Herein, hollowchannel technique was integrated with lab-on-paper for the simultaneous determination of two different concentrations of Zn~(2+) based on the origami principle, in which microfluidic channels were first patterned on a cellulose paper using commercial solid-state wax printer. Hollow-channels were created by laser cutting method as the role of both injecting ending and reaction tank. After screen printing three electrodes system, the resulting planar paper sheets were then folded into steric structures and functionalized by in-situ synthesized reduced graphene oxide. As a proof-of-concept, such lab-on-paper device was employed in the ratiometric electrochemical monitoring of zinc ion from the environment and HepG2 cells extract, by combining with co-catalysis of porous metal-organic frameworks and hemin/G-quadruplex toward H_2O_2 in the linear range of 0.1–7,000 nmol/L. The results indicated that integrating hollow-channel with steric lab-on-paper offered a new methodological approach for the development of metal ions monitoring research. It is believed that it could be useful for various point-of-care related research fields, such as, on-site environmental monitoring, food safety, and disease diagnosis. The construction of flexible platform possessing the functions of immobilizing, separating, rinsing, and high-throughput analysis plays a significant role in biological and clinical research. Herein, hollowchannel technique was integrated with lab-on-paper for the simultaneous determination of two different concentrations of Zn ~ (2+) based on the origami principle, in which microfluidic channels were first patterned on a cellulose paper using commercial solid-state wax printer. Hollow-channels were created by laser cutting method as the role of both injecting ending and reaction After screen printing three electrodes system, the resulting planar paper sheets were then folded into steric structures and functionalized by in-situ synthesized reduced graphene oxide. As a proof-of-concept, such a lab-on-paper device was employed in the ratiometric electrochemical monitoring of zinc ion from the environment and HepG2 cells extract, by combining with co-catalysis of porous metal-organic fr ameworks and hemin / G-quadruplex toward H 2 O 2 in the linear range of 0.1-7,000 nmol / L. The results indicated that integrating hollow-channel with steric lab-on-paper offered a new methodological approach for the development of metal ion monitoring research. It is believed that it could be useful for various point-of-care related research fields, such as, on-site environmental monitoring, food safety, and disease diagnosis.