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血小板在激活剂作用下会快速活化并释放具有高促凝活性的血小板膜微粒(PMPs),进而诱发出凝血功能障碍,但PMPs的形成机制有待明确。探针跳跃式离子电导显微镜(HPICM)具有在生理环境下对活体细胞进行非接触式实时高分辨率成像的技术优势。本文运用HPICM技术实时监测血小板在胞内钙离子诱导剂A23187和细胞松弛素D作用下活化及PMPs形成的过程,证明了胞内钙离子浓度和细胞骨架蛋白在血小板活化及PMPs形成中发挥了重要作用;并发现与扁平型血小板相比,突起型血小板对A23187和细胞松弛素D更为敏感,这对利用HPICM技术研究血小板活化与出凝血功能调节之间的关系具有指导意义。
Platelets activate rapidly and release platelet membrane microparticles (PMPs) with high procoagulant activity under the action of an activator, thereby inducing coagulation dysfunction. However, the mechanism of PMPs formation needs to be clarified. The probe-leaping ion conductance microscope (HPICM) has the technical advantage of non-contact, real-time, high-resolution imaging of living cells under physiological conditions. In this paper, real-time monitoring of platelet activation and PMPs under the action of intracellular Ca2 + -adrenergic agent A23187 and cytochalasin D was used to monitor the intracellular calcium concentration and cytoskeletal protein play an important role in platelet activation and PMPs formation And found that platelets were more sensitive to A23187 and cytochalasin D than platelet-rich platelets, which is instructive in using HPICM to study the relationship between platelet activation and coagulation regulation.