如今,越来越多的非病毒基因载体被应用于基因传递中,聚乙烯亚胺(PEI)作为一种重要的阳离子聚合非病毒基因传递载体受到广泛的关注。针对如何克服其生物不可降解性和细胞毒性并进一步提高转染效率的问题,笔者通过文献综合介绍了一些新的策略,并具体对以PEI为基础的新型载体的合成、新型体外转染方法的建立以及体内应用等方面的研究进展进行综述。 Nowadays, more and more non-viral gene vectors are used in gene delivery. Polyethyleneimine (PEI) has attracted extensive attention as an important cationic polymeric non-viral gene delivery vector. Aiming at the problem of how to overcome its non-biodegradability and cytotoxicity and further improve the transfection efficiency, the author introduces some new strategies through the literature, and further elaborates on the synthesis of new PEI-based vectors and the novel in vitro transfection methods Establishment and in vivo application of research progress are reviewed.