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采用毛笔挑取均匀一致的末龄韭菜迟眼蕈蚊幼虫 1 0～ 2 0头 ,应用浸渍法将供试幼虫在药剂中浸渍 30s后 ,移到葱白上 2 0～ 2 2℃培养 48h ,以毛笔触虫体 ,幼虫不动为死亡 ,作为该虫室内毒力测定方法。此法简便实用 ,稳定性和重复性好。
Using a brush to pick uniform larvae of the last instar larvae, 10 to 20 larvae of M. gondii were immersed in the agent for 30s by dipping method and then transferred to a shalloterid medium for culturing at 20-2 2 ° C for 48 hours to Brush stylus body, the larvae immobile death, as the indoor toxicity determination of insects. This method is simple and practical, good stability and repeatability.