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Tb3+和沙拉沙星(SRFX)反应生成二元配合物,发射Tb3+的特征荧光。人血清白蛋白(HSA)能够增强Tb3+-SRFX配合物的荧光强度,据此,建立了荧光法测定HSA的新方法。在最佳测定条件下,当HSA的浓度在0.50～90.0 mg/L时,Tb3+-SRFX-HSA体系荧光强度的增强和HSA的浓度有良好的线性关系,方法的检出限为0.13 mg/L。用该方法测定了人血清中HSA的含量,回收率在99.2%～99.6%之间。同时对荧光强度增强的机理进行了探讨。
Tb3 + reacts with sarafloxacin (SRFX) to form binary complexes that emit characteristic fluorescence of Tb3 +. Human serum albumin (HSA) can enhance the fluorescence intensity of Tb3 + -SRFX complex, therefore, a new fluorescence spectrometry method for the determination of HSA was established. Under the optimal conditions, the fluorescence intensity of Tb3 + -SRFX-HSA increased linearly with the concentration of HSA when the concentration of HSA was between 0.50 and 90.0 mg / L. The detection limit was 0.13 mg / L . The HSA content in human serum was determined by this method. The recoveries ranged from 99.2% to 99.6%. At the same time, the mechanism of enhanced fluorescence intensity was discussed.