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癌症的攻克和预防需要更为灵敏和特异的早期诊断和术后控制标志物.基于生物质谱的定量蛋白质组学技术由于灵敏度高、分析速度快、通量高,在肿瘤标志物的筛查中得到了广泛的应用.终端等重同位素标记串级质谱定量方法,通过对肽段的N端和C端进行互补的同位素标记产生等重标记的肽段,在一级质谱中呈现相同的质荷比,而在串级质谱中产生碎片离子对用于蛋白质的定性和定量.该方法以其简单的操作、高准确度和高通量的优势为生物标志物的发现提供了多种新的思路,为肿瘤标志物筛查提供新的手段.本文综述了终端等重同位素标记串级质谱定量方法的最新发展及其在生物标志物研究中的应用.
Cancer capture and prevention require more sensitive and specific early diagnosis and postoperative control markers Biomass-based quantitative proteomics technology due to high sensitivity, high speed, high throughput, in the screening of tumor markers Has been widely used.Heterogeneous isotope tandem mass spectrometry tandem mass spectrometry quantitative method, by peptide N-terminal and C-terminal complementary isotope labeling generated equal weight markers in the first mass spectrum showed the same mass , And fragment ion pairs are generated in tandem mass spectrometry for the qualitative and quantitative determination of proteins.This method offers many new ideas for the discovery of biomarkers with its simple operation, high accuracy and high throughput , Provide a new method for tumor marker screening.This review summarizes the latest developments of the quantitative method for the determination of terminal iso-isotope labeled tandem mass spectrometry and its application in biomarker research.