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棉花黄萎病是一种土传病害,了解黄萎病菌微菌核在土壤中的密度、分布、消长等情况,对该病的深入研究有着重要的意义。国外报道检验土壤中黄萎病菌微菌核的方法,其关键都是使用选择性培养基。但所用试剂,或因价格昂贵,或因国内没有,或因使用效果不理想,杂菌干扰大,微菌核出现频率低,菌落不明显等等原因,而无法应用。国内又无这方面研究的报道,因此,我们从1983—1985年进行研究,现研
Verticillium dahliae is a soil-borne disease. Understanding the density, distribution, growth and decline of soil-borne bacteria in Verticillium dahliae is of great significance to the further study of this disease. Foreign reports to test soil microbial pathogen Verticillium wilt method, the key is the use of selective medium. However, the reagents used can not be used due to the high cost, the lack of domestic use, the unsatisfactory effect of use, the interference of bacteria, the low frequency of microscopic bacterial nuclei, the inconspicuous colonies and the like. There is no report on the research in this field in our country. Therefore, we did research from 1983 to 1985,