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1971年在日本山形县庄内地方稻瘟病严重发病的地区,观察到春雷霉素(以下简称KSM)效果差的事实,而且在山形县农业试验场庄内分场的迟播苗试验田也看到有同样的现象。其原因推想不是由于药剂不好或者使用方法的错误,而是由于耐药菌的出现。1971年秋从庄内地方KSM效果降低的地区采集分离的8株菌株的稻瘟病菌,用添加KSM的稻草煎汁洋菜培养基(PH5.0),测定菌落的生长,鉴定对KSM的敏感性。结果全部菌株对KSM
In 1971, in Japan, Yamagata prefecture, where rice blast was severely affected, the effect of kasugamycin (hereinafter referred to as KSM) was not observed. In late rice sowing experiment field in Yamagata Prefecture Agricultural Experiment Station, The phenomenon. The reason is not supposed to be due to poor medicine or the use of methods of error, but because of the emergence of drug-resistant bacteria. In the autumn of 1971, eight isolates of Magnaporthe grisea were collected from the area where the KSM effect was reduced in Zhuangzi. The growth of the colonies was determined by using KSM-containing Fritillaria ussuriensis (PH5.0), and the sensitivity to KSM was determined. Results All strains against KSM