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作者自１９９４年１月开始采用抽空房水Ｃ３Ｆ８惰性气体充填前房，以肾胺和低分子右旋醣酐等量混合液制成的角膜保存液作湿房，于４．０℃下保存兔眼球８只，人眼２只，保存期１－７天。以锥蓝角膜内皮染色、温度逆转试验及扫描电镜检测各保存期的角膜内皮活性，证明保存５天以内的角膜内皮细胞存活率达９０％以上；温度逆转试验的角膜变薄值达５５μｍ以上；角膜内皮细胞形态正常。采用上法保存５天的人角膜作４例部分穿透角移术，术后观察平均９个月，植片全部透明。该法简单易行，不易污染，值得进一步研究、完善、推广。
The authors began filling the anterior chamber with evacuated aqueous C3F8 inert gas from January 1994 to a wet room with a corneal preservation solution made of a mixture of equal amounts of renal amine and low molecular weight dextran and preserved at 4.0 ° C Eyeball 8, human eye 2, shelf life of 1-7 days. The corneal endothelium activity of corneal endothelial cells was preserved after stored for 5 days with the staining of cone blue corneal endothelial cells, temperature reversal test and scanning electron microscopy. The corneal thinning value of temperature reversal test was more than 55μm. Corneal endothelial cells in normal form. Using the method of preservation of human corneas for 5 days for 4 cases of partial penetrating angle shift, the average postoperative observation of 9 months, all the transparent graft. The method is simple, easy to pollution, it is worth further study, improvement, promotion.