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不同来源的活性羰基化合物(主要是非酶糖基化和脂质过氧化中间产物)能和多种蛋白发生交联反应,导致其结构的改变及功能的丧失.利用小牛血清白蛋白/丙二醛(BSA/MDA)这一蛋白羰基应激模式,检测不同浓度的MDA对BSA吸光和荧光的影响.同时,通过向BSA/MDA反应体系加入不同浓度的维生素B1(VB1),检测VB1对蛋白羰基修饰的抑制作用.实验结果表明,蛋白的羰基修饰生成了老年色素类荧光物质(APFs),同时使蛋白的羰基含量增加;VB1在一定程度上抑制了蛋白羰基含量的增加.
Activated carbonyl compounds (mainly non-enzymatic glycosylation and lipid peroxidation intermediates) from different sources can cross-react with various proteins, resulting in structural changes and loss of function. The use of bovine serum albumin / (BSA / MDA), a protein carbonyl stress mode, was used to detect the effects of different concentrations of MDA on the absorbance and fluorescence of BSA.At the same time, different concentrations of vitamin B1 (VB1) were added to the BSA / MDA reaction system to detect the effect of VB1 on the protein Carbonyl modification.Experimental results showed that the carbonyl modification of the protein generated the aging pigment fluorescent substances (APFs), while increasing the carbonyl content of the protein; VB1 to some extent inhibited the increase of the carbonyl content of the protein.