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以胶原纤维(CF)接枝杨梅单宁(BT)为载体,制备了一种新型纳米银(nAg)抗菌剂(CF-BTx-nAg).BT为一种天然的酚类化合物,将其接枝到胶原纤维上,可提高Ag的负载量、控制纳米Ag的粒径及分布.通过扫描电镜(SEM)、红外光谱(FTIR)、透射电镜(TEM)、X射线衍射(XRD)、X射线光电子能谱(XPS)等手段对该抗菌剂的物化性能进行表征,发现该抗菌剂具有规则的纤维结构,纳米Ag颗粒的平均粒径为3.25 nm且分散均匀.抗菌实验结果表明,不同BT接枝度的CF-BTx-nAg对大肠杆菌均具有较好的抑制能力,其中CF-BT0.2-nAg的抑制能力最强.当CF-BT0.2-nAg与～105cfu/mL的大肠杆菌菌悬液在310 K、150 r/min下连续培养(Ag质量浓度为8 mg/L),培养4 h后已检测不到活细菌的存在.CF-BT0.2-nAg对大肠杆菌的最低抑菌质量浓度(MIC)≤4mg Ag/L,且CF-BT0.2-nAg至少可重复使用5次,表现出优良的抗菌性能.
A new type of nano-Ag (nAg) antibacterial agent (CF-BTx-nAg) was prepared by using collagen (CF) grafted bayberry tannin (BT) as support.BT is a kind of natural phenolic compound, The effect of Ag on the collagen fibers can increase the loading of Ag, and control the size and distribution of nano-Ag.METHODS, SEM, FTIR, TEM, X-ray diffraction (XRD) Photoelectron spectroscopy (XPS) and other means of the physical and chemical properties of the antibacterial agent was characterized and found that the antibacterial agent has a regular fiber structure, the average particle size of nano-Ag particles 3.25 nm and evenly dispersed antibacterial experimental results show that different BT The degree of inhibition of CF-BTx-nAg was better than that of E. coli CF-BT0.2-nAg.When the CF-BT0.2-nAg and ~ 105cfu / mL Escherichia coli The suspension was continuously cultured at 310 K and 150 r / min (Ag concentration of 8 mg / L), and no viable bacteria could be detected after 4 h culture.CFC-BT0.2-nAg had the lowest inhibitory effect on Escherichia coli Bacteria mass concentration (MIC) ≤ 4mg Ag / L, and CF-BT0.2-nAg can be reused at least 5 times, showing excellent antibacterial properties.