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目的：检测多药耐药相关蛋白（ＭＲＰ）基因在乳腺癌组织中的表达水平，并和谷胱甘肽Ｓ－转移酶－ｐｉ（ＧＳＴ－π）的表达作相关性比较，探讨其在临床的应用价值。方法：用定量ＲＴ－ＰＣＲ技术，检测了５５例乳腺癌组织中ＭＲＰ和其中４０例ＧＳＴ－πｍＲＮＡ的表达水平，并和术前化疗比较分析。结果：ＭＲＰ表达的阳性率为７６．４％，高表达占１８．２％，中低表达各占２３．６％和３４．５％。ＭＲＰ和ＧＳＴ－π有４０％的共表达率，虽然ＭＲＰ表达阳性率及水平和术前化疗关系不明显，但化疗后二者表达有正相关趋势。结论：ＭＲＰ在乳腺癌中普遍表达，以中低表达为主，其和ＧＳＴ－π的共表达可能和化疗药物诱导有关。
OBJECTIVE: To detect the expression level of multidrug resistance-associated protein (MRP) gene in breast cancer tissues and to compare the correlation with glutathione S-transferase-pi (GST-π) expression and to explore its clinical significance. The application value. METHODS: Quantitative RT-PCR was used to detect the expression of MRP in 40 cases of breast cancer and 40 cases of GST-π mRNA, and compared with preoperative chemotherapy. Results: The positive rate of MRP expression was 76.4%, the high expression accounted for 18.2%, and the middle and low expression accounted for 23.6% and 34.5% respectively. There was a 40% co-expression rate of MRP and GST-π. Although the positive rate and level of MRP expression were not significantly related to preoperative chemotherapy, there was a positive correlation between the expression of MRP and GST-π after chemotherapy. Conclusion: MRP is predominantly expressed in breast cancer, mainly in medium and low expression. The co-expression of GST-π may be related to the induction of chemotherapy drugs.