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用ＰＣＲ（聚合酶链反应）及序列特异性寡核苷酸探针杂交技术（ＳＳＯＰＨ）对中国北方人中ＨＬＡ－ＤＲＢ１和ＤＱ基因与ＩＤＤＭ的遗传易感相关性联合进行了研究，以５４例胰岛素依赖型糖尿病患儿为研究对象，４０例正常成年供血员为对照。结果表明：ＤＲＢ１＊０３０１、ＤＱＡ１＊０５０１、０３０１和ＤＱＢ１＊０２０１为ＩＤＤＭ易感性基因，ＤＱＡ１＊０１０３、ＤＱＢ１＊０６０１为ＩＤＤＭ保护性基因。ＤＲＢ１＊０３０１－ＤＱＡ１＊０５０１－ＤＱＢ１＊０２０１为ＩＤＤＭ易感性单倍型。ＤＲ３／ＤＲ４和ＤＲ３／ＤＲ９杂合子在病人中显著增高，表明ＤＲ３和ＤＲ４或ＤＲ３和ＤＲ９单倍型的易感性效果之间产生了协同作用。
The genetic susceptibility to HLA-DRB1 and DQ genes in Chinese northern populations was studied by polymerase chain reaction (PCR) and sequence-specific oligonucleotide probe hybridization (SSOPH) in 54 patients Children with insulin-dependent diabetes mellitus as study subjects, 40 normal adult blood donors as a control. The results showed that: DRB1 * 0301, DQA1 * 0501,0301 and DQB1 * 0201 are IDDM susceptibility genes, DQA1 * 0103 and DQB1 * 0601 are IDDM protective genes. DRB1 * 0301-DQA1 * 0501-DQB1 * 0201 is an IDDM susceptibility haplotype. Heterozygotes for DR3 / DR4 and DR3 / DR9 were significantly elevated in patients, suggesting a synergistic effect between the susceptibility effects of DR3 and DR4 or DR3 and DR9 haplotypes.