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将3种肿瘤细胞搭载于“神舟4号”的卫星返回舱内,经过7天太空飞行,回收后对存活细胞进行单克隆化,观察细胞形态,并测定了细胞周期、黏附力及细胞因子表达.结果显示,经太空飞行,小鼠黑色素瘤B16细胞的周期发生改变,G1期细胞明显增多(p<0.05),并表现多种细胞形态;人肺鳞癌细胞L78对血管内皮细胞黏附力明显减弱,但经传代培养其黏附力恢复且超过对照组细胞;Caski细胞IL-2、IL-8、TNF和TGF的表达均明显增加,而L78细胞上述4种细胞因子的表达均显著下降.结论,太空环境可影响肿瘤细胞的某些生理特性,但可否影响肿瘤细胞的免疫原性,仍需做进一步的实验.
The three kinds of tumor cells were carried in the satellite capsule of “Shenzhou 4”. After 7 days of space flight, the surviving cells were recovered after being monoclonated to observe the cell morphology and cell cycle, adhesion and cytokine expression The results showed that in the space flight, the cycle of mouse melanoma B16 cells changed, the number of G1 phase cells increased significantly (p <0.05), and showed a variety of cell morphology; human lung squamous cell carcinoma L78 adhesion to vascular endothelial cells significantly But the expression of IL-2, IL-8, TNF and TGF in Caski cells were significantly increased, while the expression of the above four cytokines in L78 cells were significantly decreased.Conclusion , The space environment can affect some physiological characteristics of tumor cells, but can affect the immunogenicity of tumor cells, still need to do further experiments.