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根据筛选到的与桃果实非酸/酸性状紧密连锁的3个AFLP标记特异片段序列信息,设计特异引物BFPA1/A2、BFPB1/B2和BFPC1/C2。3个特异引物在‘京玉’桃、‘美味’油桃及其正反交F1代69株群体中的PCR检测结果表明:引物BFPB1/B2在果实性状表现为酸的后代个体中扩增出分子量大小为158bp的特异条带,且根据特异片段AT-CTA199不同部位设计的引物都能稳定扩增,只是片段大小有所差异;特异扩增检测F1群体分离结果与原先AFLPAT/CTA标记完全一致,表明AFLP标记已成功转化为SCAR标记,该SCARBFPB1/B2标记与D基因的连锁距离为2.99cM。利用SCARBFPB1/B2标记对‘大久保’桃ב兴津’油桃的F2群体60株个体的果实非酸/酸性状进行检测,基因型与表型性状符合率为96.7%,并且表现为共显性标记。特异引物BFPA/A和BFPC/C的扩增多态性条带在亲本及后代中消失。
According to the sequence information of three AFLP markers closely linked to the non-acid / acid traits of peach fruit, three specific primers BFPA1 / A2, BFPB1 / B2 and BFPC1 / C2 were designed in ’Jingyu’ The PCR results of 69 delicious F1 nectarines and reciprocal cross F1 progenies showed that the BFPB1 / B2 primers amplified a 158bp specific band in progeny individuals with fruit traits, The primers designed for different regions of AT-CTA199 could amplify stably except for the size of the fragments. The result of the specific amplification assay of F1 population was completely consistent with the original AFLPAT / CTA marker, indicating that the AFLP marker has been successfully transformed into the SCAR marker. The linkage distance between the SCARBFPB1 / B2 marker and the D gene was 2.99cM. The non-acid / acid traits of 60 individuals from the F2 population of ’Okubo’ × ’Xingjin’ nectarines were detected using SCARBFPB1 / B2 marker. The coincidence rate of genotypes and phenotypic traits was 96.7% Sexual markers. The amplified polymorphic bands of specific primers BFPA / A and BFPC / C disappeared in the parents and offspring.