目的探讨新疆地区孕中期羊水细胞培养影响因素。方法该实验室对1 378例具有产前诊断指征的孕妇行羊膜腔穿刺,无菌操作下羊水穿刺获得孕中期羊水14mL,接种T瓶培养7~9d。及时处理不合格羊水,规范培养环境,严格要求无菌操作。采用消化法收获细胞,常规染色体制片后进行产前诊断,分析核型。结果通过消化法培养1 378例羊水细胞,失败13例,成功1 365例,成功率99.1%。结论羊水细胞培养各个环节至关重要,直接影响培养结局;符合产前诊断适应征孕妇的异常率高于正常孕妇,应指导其行侵入性产前诊断,确定胎儿核型。 Objective To investigate the influencing factors of amniotic fluid cell culture in the second trimester of Xinjiang. Methods A total of 1 378 pregnant women with prenatal diagnosis were enrolled in this laboratory. Amniotic fluid puncture under aseptic technique was used to obtain 14 mL second trimester amniotic fluid and inoculated with T bottles for 7-9 days. Promptly deal with unqualified amniotic fluid, regulate the training of the environment, strictly require aseptic operation. Cells were harvested by digestion and prenatal diagnosis was performed after conventional chromosome preparation. The karyotypes were analyzed. Results A total of 1 378 cases of amniotic fluid cells were cultured by digestion, and 13 cases failed. One hundred and sixteen cases were successfully treated with a success rate of 99.1%. Conclusions Amniotic fluid cell culture is crucial in all aspects of culture, which directly affects the outcome of culture. The anomalous rate of prenatal diagnosis of pregnant women is higher than that of normal pregnant women. The prenatal diagnosis of invasive amniotic fluid should be guided to determine the fetal karyotype.