论文部分内容阅读
目的对温州市及附近地区人群进行广州管圆线虫病血清流行病学调查。方法以广州管圆线虫Ⅴ期幼虫全抗原为包被抗原,采用间接酶联免疫吸附试验(ELISA)检测随机抽取的温州市(温州医学院)及附近地区(乐清、瓯海、永嘉、泰顺)人群血清中广州管圆线虫特异性抗体。结果温州地区人群血清广州管圆线虫抗体总阳性率为4.32%(35/810),其中温州医学院、瓯海、永嘉、泰顺地区人群血清阳性率分别为2.78%(5/180)、2.40%(3/125)、3.68%(7/190)和14.08%(20/142),乐清地区未检测到阳性标本(0/173)。有食生或半生螺史者抗体阳性率(37.63%)高于无食生螺肉史者(0)。结论温州市区及附近地区存在血清广州管圆线虫抗体阳性人群。广州管圆线虫感染可能与不洁饮食有关。
Objective To investigate the serological epidemiology of angiostrongyliasis in Guangzhou and its neighboring areas. Methods The antigen of phase Ⅴ of larvae of Acanthamoeba beijng was coated with antigens. Indirect enzyme-linked immunosorbent assay (ELISA) was used to detect the plasma levels of Wenzhou (Wenzhou Medical College) and nearby areas (Yueqing, Ouhai, Yongjia, Taishun ) Serum of C. elegans in the serum of C. elegans specific antibodies. Results The positive rate of serogroup A in Guangzhou was 4.32% (35/810). The positive rates of seroprevalence in Wenzhou Medical College, Ouhai, Yongjia and Taishun were 2.78% (5/180) and 2.40 % (3/125), 3.68% (7/190) and 14.08% (20/142) respectively. No positive samples were detected in Yueqing area (0/173). The positive rate of antibodies (36.63%) was higher than those without food history (0). Conclusions There is a serum-positive seronegatans population in Wenzhou and its vicinity. Infection may be related to unclean diet.