本文将结合态环己醇提出在酸性下使之游离,然后用气相色谱法测定。材料和方法试剂环己酮、环庚醇(内标)、环己醇等。尿样的采集收集27名生产录像带厂工人班末尿样,于0℃以下冷冻保存。这些工人接触环己酮的时间加权平均浓度(TWA)为2～30 ppm。尿中酚类化合物于-20℃,不加防腐剂可稳定20天。样品处理取尿样2 ml,加浓盐酸0.4ml,95℃加热90分钟以水解尿中环己醇结合物。冷却后加0.4 g NaCl,在分液漏斗中用4 In this paper, in combination with cyclohexanol, it is proposed to make it free under acidic conditions and then determined by gas chromatography. Materials and Methods Reagents cyclohexanone, cycloheptanol (internal standard), cyclohexanol and so on. Collection of urine samples collected in the production of video tape factory workers in the last class of urine samples, frozen below 0 ℃. The time-weighted average exposure (TWA) of these workers to cyclohexanone ranged from 2 to 30 ppm. Urine phenolic compounds at -20 ℃, without preservatives can be stable for 20 days. Sample processing Urine samples were taken 2 ml, plus concentrated hydrochloric acid 0.4ml, 95 ℃ heated 90 minutes to hydrolyze urinary cyclohexanol conjugate. After cooling plus 0.4 g of NaCl, in a separatory funnel with 4