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目的研究慢性乙型肝炎及肝炎肝硬化患者外周血淋巴细胞亚群及自然杀伤(NK)细胞的变化。方法应用流式细胞术检测对照组、慢性乙型肝炎(慢乙肝组)及肝炎肝硬化(肝硬化组)患者外周血淋巴细胞亚群CD3+、CD3+CD4+、CD3+CD8+、NK细胞、CD19+(B细胞)表达百分比。结果慢乙肝组患者CD3+CD4+及NK细胞百分比降低,B细胞百分比增加,与对照组相比差异有统计学意义。肝硬化组患者CD3+、CD3+CD4+、CD3+CD8+及NK细胞百分比均明显下降,B细胞百分比及CD4+/CD8+增高,与对照组相比差异有统计学意义。结论慢性乙型肝炎患者体内存在体液免疫和细胞免疫功能紊乱。外周血淋巴细胞免疫分型检测对于了解患者免疫状态,判别病情及预后有一定临床意义。
Objective To study the changes of peripheral blood lymphocyte subsets and natural killer (NK) cells in patients with chronic hepatitis B and liver cirrhosis. Methods Flow cytometry was used to detect the expression of CD3 +, CD3 +, CD4 +, CD3 +, CD8 +, CD19 + (+) in peripheral blood lymphocytes in patients with chronic hepatitis B (chronic hepatitis B) and cirrhosis B cells) percentage of expression. Results The percentage of CD3 + CD4 + and NK cells and the percentage of B cells in chronic hepatitis B group were significantly lower than those in control group. The percentage of CD3 +, CD3 +, CD4 +, CD3 + CD8 + and NK cells in cirrhosis patients were significantly decreased, while the percentage of B cells and CD4 + / CD8 + increased significantly compared with the control group. Conclusion There are humoral and cellular immune dysfunction in patients with chronic hepatitis B. Peripheral blood lymphocyte immunophenotyping test for understanding the patient’s immune status, determine the disease and prognosis of a certain clinical significance.