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目的:探讨治疗糖尿病中药复方对早期糖尿病小鼠的作用机制并对各方进行筛选。方法:造模前禁食13 h,一次性腹腔注射链脲佐菌素170 mg·kg~(-1)造模成糖尿病小鼠(血糖≥13.6 mmol·L~(-1)),并随机分为糖尿病模型组(DM),DM-1治疗组,DM-2治疗组,DM-3治疗组,DM-4治疗组,DM-5治疗组,DM-6治疗组。2周后,检测各组血清及肾组织一氧化氮(NO)、丙二醛(MDA)含量,计算肾脏器指数。结果:与糖尿病模型组相比,DM-2组肾脏指数降低最为显著,抑制血清NO作用最强;DM-2、DM-5组肾组织NO含量显著降低;DM-2、DM-4、DM-6组血清MDA含量明显降低;DM-2、DM-3、DM-4组肾组织MDA含量降低最显著。结论:糖尿病早期给予地知复方的不同组方有一定的治疗作用,它们具有不同程度的抑制肾脏肥厚增生,抗氧化,抑制高血糖刺激引起的NO升高作用,但是只有方2对上述氧化应激指标的改善均有作用,且作用最强。
Objective: To explore the mechanism of treatment of diabetes mellitus in early diabetic mice and screen all the patients. Methods: The rats were fasted for 13 hours before model making. Diabetic mice (blood glucose> 13.6 mmol·L -1) were injected intraperitoneally with streptozotocin (170 mg · kg -1) The rats were divided into diabetic model group (DM), DM-1 treatment group, DM-2 treatment group, DM-3 treatment group, DM-4 treatment group, DM-5 treatment group and DM-6 treatment group. Two weeks later, the contents of nitric oxide (NO) and malondialdehyde (MDA) in serum and kidney of each group were measured to calculate the index of kidney organ. Results: Compared with diabetic model group, the index of kidney in DM-2 group was the most significant decrease and NO in serum was the strongest. The content of NO in DM-2 and DM-5 group was significantly lower than that in DM group The level of MDA in the serum of -6 group was significantly lower than that of the control group. The content of MDA in the kidney of DM-2, DM-3 and DM-4 group was the most significant. CONCLUSION: The different prescriptions given to Di Zhi in early stage of diabetes have a certain therapeutic effect. They have different degrees of inhibition on renal hypertrophy, anti-oxidation and inhibition of NO-induced elevation of hyperglycemia. However, Indispensable indicators of improvement have a role, and the strongest.