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目的:探讨赫赛汀联合姜黄素对HER2阳性卵巢癌细胞系SKOV3增殖能力的影响。方法:体外培养至对数生长期的人卵巢癌细胞系SKOV3细胞分为对照组、赫赛汀治疗组、姜黄素治疗组以及赫赛汀联合黄素治疗组,利用四唑盐(MTT)比色法和平板集落形成试验比较各组细胞增殖能力,利用AnnexinV-FITC/PI染色比较各组细胞凋亡。结果:赫赛汀联合姜黄素治疗组SKOV3细胞活力明显低于对照组及单一药物处理组,平板集落形成试验结果表明各组细胞集落形成率分别为83.4%、36.8%、59.2%和24.7%,赫赛汀联合姜黄素处理组SKOV3细胞集落形成能力显著低于另外3组(P<0.01),Annexin V-FITC/PI染色并用流式细胞仪分析表明各组SKOV3细胞早期凋亡率分别为1.3%、26.4%、9.3%和39.1%,赫赛汀联合姜黄素处理组细胞早期凋亡率显著低于其余3组(P<0.01)。结论:赫赛汀联合姜黄素能够抑制卵巢癌细胞系SKOV3的增殖,这种抑制作用是通过促进肿瘤细胞凋亡实现的。
Objective: To investigate the effect of Herceptin combined with curcumin on the proliferation of HER2-positive ovarian cancer cell line SKOV3. METHODS: Human ovarian cancer cell line SKOV3 cells cultured in vitro to logarithmic growth phase were divided into control group, Herceptin treatment group, curcumin treatment group and Herceptin combined with flavin treatment group. MTT ratio Colorimetric assay and plate colony formation assay were used to compare the cell proliferative ability of each group. Cell apoptosis was compared with Annexin V-FITC / PI staining. Results: The cell viability of SKOV3 cells treated with Herceptin plus curcumin was significantly lower than that of the control and single drug treatment groups. The results of colony formation assay showed that the colony formation rate of each group was 83.4%, 36.8%, 59.2% and 24.7%, respectively. The colony forming ability of SKOV3 cells treated with Herceptin combined with curcumin was significantly lower than that of the other three groups (P <0.01). Annexin V-FITC / PI staining and flow cytometry analysis showed that the early apoptotic rates of SKOV3 cells in each group were 1.3 %, 26.4%, 9.3% and 39.1%. The early apoptotic rate of Herceptin plus curcumin group was significantly lower than that of the other three groups (P <0.01). Conclusion: Herceptin combined with curcumin can inhibit the proliferation of ovarian cancer cell line SKOV3, and this inhibition is achieved through the promotion of tumor cell apoptosis.