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利用抗 HGV E2区的 3株单克隆抗体 M6、M1 3、M30作为筛选配基 ,对随机 6肽库进行亲和筛选 . 3轮筛选的投入产出比逐轮升高至 3.5× 1 0 -3、假阳性率逐轮降低至 0 .4% ,提示具有良好的富集效果 .从第 3轮随机挑出 1 2个克隆进行功能鉴定 ,结果表明 8个克隆与 M6抗体有较强的特异性结合力并有较好的竞争抑制作用 ,测序发现它们的外源肽具有核心序列 :WA( W/Y) WXH,该序列与 HGV同源性低 ;用外源肽与核心序列相似的噬菌体克隆 P6GC9做竞争抑制试验 ,约 3×1 0 10个噬菌体即可较好地抑制 M6单抗与 HGV抗原结合 .该多肽可能是 HGV E2区识别 M6单抗并具有一定功能的模拟表位 .
HGV E2 region using three monoclonal antibodies M6, M1 3, M30 as screening ligand random peptide library 6 affinity screening three rounds of screening input-output than the round by 3.5 × 10 ~ 3, False positive rate reduced to 0.4% by wheel, suggesting a good enrichment effect.From the third round randomly selected 12 clones for functional identification, the results showed that eight clones and M6 antibodies have strong specificity The results of sequencing showed that the exogenous peptide had the core sequence WA (W / Y) WXH, which had low homology with HGV. Using phage with exogenous peptide similar to the core sequence Clone P6GC9 competitive inhibition test, about 3 × 10 10 phages can better inhibit the M6 monoclonal antibody and HGV antigen binding.The polypeptide may be HGV E2 M6 monoclonal antibody recognition and functional mimotopes.