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小鼠注射猪苓多糖和氢化考的松7天后,取其腹腔巨噬细胞,进行细胞化学的定性及定位;并用 MPV-2型显微分光光度计进行定量研究。发现应用氢化考的松后,细胞内的 AcPase、ATPase 和 ANAE 活性下降,多糖含量减少。注射猪苓多糖以后,ATPase、AcPase 和 ANAE 活性增强,多糖含量显著增多。猪苓多糖和氢化考的松同时注射,酶活性及多糖含量与对照组无明显区别。
After the mice were injected with polyporus polysaccharides and hydrocortisone for 7 days, their peritoneal macrophages were taken for the characterization and localization of the cytochemistry, and were quantitatively studied with an MPV-2 microspectrophotometer. After application of hydrocortisone, the intracellular AcPase, ATPase, and ANAE activity decreased, and the polysaccharide content decreased. After injection of polyporus polysaccharides, the activity of ATPase, AcPase and ANAE increased, and the polysaccharide content increased significantly. Simultaneous injection of polyporus umbellatus polysaccharide and hydrocortisone resulted in no significant difference between the enzyme activity and polysaccharide content in the control group.