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目的：检测大肠腺瘤及腺癌中Ｐ５３蛋白及Ｂｃｌ－２蛋白的表达，探讨ｐ５３基因、ｂｃｌ－２基因在大肠癌发生发展过程中的作用。方法：应用免疫组织化学ＡＢＣ法检测１０例正常大肠粘膜、３０例大肠腺瘤、１００例大肠腺癌及癌旁粘膜中Ｐ５３蛋白及Ｂｃｌ－２蛋白的表达。结果：大肠腺瘤Ｐ５３、Ｂｃｌ－２蛋白阳性率分别为２６．７％及７６．７％，大肠腺癌Ｐ５３、Ｂｃｌ－２蛋白阳性率分别为４３％及６７％，均显著高于正常粘膜与癌旁粘膜（Ｐ＜０．０１）；随着腺瘤不典型增生程度的加重，Ｐ５３蛋白阳性率显著增高（Ｐ＜０．０１），而Ｂｃｌ－２蛋白表达与腺瘤分级无关；大肠腺癌Ｐ５３蛋白阳性率显著高于腺瘤Ⅰ级不典型增生（Ｐ＜０．０１），与Ⅱ、Ⅲ级不典型增生间差异无统计学意义（Ｐ＞０．０５），分化差的大肠腺癌Ｐ５３蛋白阳性率显著高于分化好的大肠腺癌（Ｐ＜０．０１），而分化差的大肠腺癌Ｂｃｌ－２蛋白阳性率显著低于分化好的大肠腺癌（Ｐ＜０．０５），大肠腺癌Ｐ５３、Ｂｃｌ－２蛋白表达呈负相关（Ｐ＜０．０５）；Ｐ５３蛋白表达患者复发率高、５年生存率低（Ｐ＜０．０５），Ｂｃｌ－２蛋白过度表达的大肠癌患者复发率低、５年生存率高（Ｐ＜０．０５）。结论：ｂｃｌ－２?
Objective: To detect the expression of P53 protein and Bcl-2 protein in colorectal adenoma and adenocarcinoma, and to explore the role of p53 gene and bcl-2 gene in the development of colorectal cancer. Methods: Immunohistochemistry ABC method was used to detect the expression of P53 protein and Bcl-2 protein in 10 cases of normal colorectal mucosa, 30 cases of colorectal adenoma, and 100 cases of colorectal adenocarcinoma and paraneoplastic mucosa. Results: The positive rates of P53 and Bcl-2 protein in colorectal adenoma were 26.7% and 76.7%, respectively. The positive rates of P53 and Bcl-2 protein in colorectal adenocarcinoma were 43% and 67%, respectively, which were significantly higher than those in normal mucosa. With paraneoplastic mucosa (P <0.01); As the adenoma aggravated, the positive rate of P53 protein was significantly higher (P <0.01), and Bcl-2 protein expression was not associated with the grade of adenoma; Large intestine The positive rate of P53 protein in adenocarcinoma was significantly higher than that in adenoma grade I dysplasia (P<0.01), and there was no significant difference between grade II and grade III dysplasia (P>0.05). The positive rate of P53 protein in adenocarcinoma was significantly higher than that in differentiated colorectal adenocarcinoma (P<0.01). The positive rate of Bcl-2 protein in poorly differentiated colorectal adenocarcinoma was significantly lower than that in differentiated colorectal adenocarcinoma (P<0. 05) There was a negative correlation between P53 and Bcl-2 protein expression in colorectal adenocarcinoma (P<0.05); P53 protein expression showed high recurrence rate and low 5-year survival rate (P < 0.05). 0.05), the recurrence rate of patients with colorectal cancer overexpression of Bcl-2, 5-year survival rate (P <0.05). Conclusion: bcl-2?