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目的探讨乙型肝炎表面抗原（HBsAg）弱反应性标本的检测及解决方法。方法酶联免疫吸附实验(ELISA)法筛查出本院2011年~2012年间39例HBsAg弱反应性标本如下：①S/CO值0.6~1.3，11例；②S/CO值1.3~2.5，15例；③S/CO值2.5~5.0；13例，以上标本均做酶联免疫吸附试验(ELISA)，美国雅培公司ARCHITECTi2000SR仪器定量及雅培确认实验，并比较结果的差异。结果本研究结果可见：①11例S/CO值在0.6~1.3区间，ELISA检测阳性6例，阴性5例；雅培发光定量和确认实验检测阳性5例，阴性6例，差异有统计学意义（<0.05）；②15例S/CO值在1.3~2.5区间，ELISA检测阳性13例，雅培发光定量和确认实验检测阳性13例；③13例S/CO值在2.5~5.0区间，ELISA检测阳性15例，雅培发光定量和确认实验检测阳性15例。结论 ELISA检验HBsAg容易出现假阳性，临床对于ELISA结果有怀疑的应进行确认试验验证，以保证检验结果的可靠性。“,”Objective To evaluate the detection and solution of hepatitis surface antigen weak reactive specimens.Methods We picked up 39 weak reactive samples of HBsAg From 2011 to 2012 by Enzyme-linked immunosorbent assay(ELISA) as fol own:①S/CO 0.6~1.3,11 cases;②S/CO 1.3~2.5,13 cases;③S/CO 2.5~5.0,15 cases,retested the above samples by ELISA and Abbot ARCHITECT i2000SR quantitative confirmation experiment ,then compare the dif erence between the results.Results From this study,we got fol owing.Results ①11cases of S/CO values in the 0.6 to 1.3 range, ELISA tested positive in 6 cases,negative 5 cases;Abbot luminescence assay quantification and confirmation tested positive in 5 cases,negative 6 cases,between which the dif erence was statistical y significant( <0.05).②15 cases of S/CO values ??in the 1.3 to 2.5 range,ELISA tested positive in 13 cases,Abbot luminous quantitative and experimental tested positive in 13 cases.③13cases of S/CO values in the 2.5 to 5.0 range,ELISA tested positive in 15 cases,Abbot luminescence assay quantification and confirmation tested positive in 15 cases.Conclusion It is very common of false positive in HBsAg serum by using ELISA.Therefore,For the ELISA results of clinical suspicion should be confirmed by confirmatory test to ensure the reliability of test results.