论文部分内容阅读
目的构建以Asc I酶切的云南省鼠疫菌PFGE图谱库并探究其流行病学意义。方法采用限制性内切酶Asc I对云南家、野鼠型及玉龙鼠疫菌进行酶切分析,并对电泳图谱进行聚类分析。结果 30株被试菌株分为15种PFGE型别(相似性系数为88.9%~100.0%)、5个簇、4个群,除404号菌与EV76归为一群,其余3个群分别为家鼠型基因群、野鼠型基因群及玉龙基因群。结论云南鼠疫菌PFGE基因型与生态型相吻合,具有一定的区域聚集性;玉龙鼠疫菌是云南独立的一个基因群。
Objective To construct a PFGE map of Yersinia pestis from Yunnan province with Asc I enzyme digestion and explore its epidemiological significance. Methods Restriction endonuclease Asc I was used to analyze the Yersinia pestis in Yunnan Province, Yunnan Province and Yulong Municipality. The electrophoresis patterns were analyzed by cluster analysis. Results Thirty strains were divided into 15 PFGE genotypes (similarity coefficient of 88.9% -100.0%), 5 clusters and 4 colonies, except for 404 and EV76 groups, the other 3 groups were home Murine gene group, wild mouse type gene group and jade dragon gene group. Conclusion PFGE genotypes of Y. pestis coincide with ecotypes and have certain regional aggregation. Yulong Y. pestis is an independent gene cluster in Yunnan.