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本文利用无血清培养的人胎成骨细胞(OB)体外模型,通过对 OB 内碱性磷酸酶(ALP)活性、胶原合成、DNA、蛋白含量及 cAMP 水平的测定,研究了生理浓度的 T_3(3,5,3′-三碘甲腺原氨酸)对 OB 生长、功能、分化的作用,结果显示:生理浓度的 T_3可显著地增加 OB内 ALP 的活性,并有时间相关关系,T_3作用96小时可显著地增加~3H-脯氨酸掺入到胶原蛋白中;然而,在96小时时期内,却未显著地改变细胞内蛋白及 DNA 的含量。这些结果表明.T_3引起 ALP活性、胶原合成的增加,不是由于促进了 OB 的增生,而是直接促进了体外培养的 OB 的分化而增加其功能。本研究提示,T_3在生理浓度下与体内骨形成有关。
In this study, the serum level of T_3 (superscript +) in cultured human fetal osteoblasts (OB) was measured in vitro by measuring the alkaline phosphatase (ALP) activity, collagen synthesis, DNA content, 3,5,3’-triiodothyronine) on the growth, function and differentiation of OB. The results showed that the physiological concentration of T_3 significantly increased the activity of ALP in OB, and there was a time-dependent relationship between T_3 and T_3 96 h significantly increased incorporation of 3H-proline into collagen; however, intracellular protein and DNA content were not significantly altered over a 96-hour period. These results indicate that T_3 induces ALP activity and that the increase of collagen synthesis is not due to the promotion of OB hyperplasia, but directly promotes the differentiation of OB cultured in vitro and increases its function. The present study suggests that T_3 is associated with bone formation in vivo at physiological concentrations.