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目的:建立高效液相色谱法测定儿泻贴中丁香酚的含量的方法。方法:色谱柱为Kromasil C_(18)(250 mm×4.6 mm,5μm);流动相:甲醇-水(55:45);流速:1.0 ml·min~(-1);检测波长280 nm。结果:丁香酚在0.27~6.70μg范围内,线性关系良好(r=0.999 9);平均回收率为99.76%,RSD为1.0l%(n=6)。结论:该检测方法可靠、准确、重复性好,可用于儿泻贴中丁香酚的含量测定。
Objective: To establish a method for the determination of eugenol in diarrhea by high performance liquid chromatography. METHODS: The chromatographic column was Kromasil C 18 (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol-water (55:45). The flow rate was 1.0 ml · min -1. The detection wavelength was 280 nm. Results: The eugenol showed a good linearity (r = 0.999 9) in the range of 0.27-6.70 μg with an average recovery of 99.76% and a RSD of 1.01% (n = 6). Conclusion: The method is reliable, accurate and reproducible. It can be used for the determination of eugenol in diarrhea.