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目的观察红参皂苷Rg5对MPP+诱导的SH-SY5Y细胞损伤的影响。方法 MTT法检测细胞活性;流式细胞术检测线粒体膜电位(ΔΨm)及细胞内活性氧族(ROS)水平;Western blotting方法分析酪氨酸羟化酶(TH)蛋白表达。结果不同浓度的红参皂苷Rg5处理组较250 mmol/L的MPP+单独处理组,SH-SY5Y细胞损伤明显改善。其中,ROS荧光强度值明显降低,ΔΨm荧光强度值明显升高(P<0.05),TH表达明显增加。结论红参皂苷Rg5对MPP+诱导细胞氧化应激具有一定的保护作用。
Objective To observe the effect of ginsenoside Rg5 on MPP + -induced SH-SY5Y cell injury. Methods MTT assay was used to detect the cell viability. Mitochondrial membrane potential (ΔΨm) and intracellular reactive oxygen species (ROS) levels were measured by flow cytometry. The protein expression of tyrosine hydroxylase (TH) was analyzed by Western blotting. Results Compared with 250 μmol / L MPP + treatment group, the injury of SH-SY5Y cells in Rg5 treated with different concentrations of ginsenoside was significantly improved. Among them, ROS fluorescence intensity was significantly decreased, ΔΨm fluorescence intensity was significantly increased (P <0.05), TH expression was significantly increased. Conclusion Red ginseng saponin Rg5 can protect MPP + -induced cell oxidative stress.