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以明胶作为载体,采用包埋技术成功地将鸡白痢抗体修饰于电极表面,制成非标记型鸡白痢免疫传感器。采用循环伏安法和交流阻抗图谱对不同修饰状态的电极进行表征。根据抗原抗体特异性结合形成的免疫复合物使敏感膜有效扩散截面积减小的特性,采用循环伏安法以铁氰化钾为分子探针间接检测鸡白痢抗原。通过试验确定鸡白痢阴阳性判定标准,Δi>2.5μA判为阳性,Δi<2.5μA判为阴性。该免疫传感器特异性强,重现性好(RSD<6%),准确性高(与国标检测方法符合率为88.9%),4℃干态放置7d,其响应电流为初始电流的90.2%。因此该免疫传感器可初步用于鸡白痢阴阳性判定和快速筛选。
Gelatin as a carrier, embedding technology to successfully scour the chicken white dysentery antibody modified electrode surface, made non-tag type diarrhea immunosensor. Cyclic voltammetry and AC impedance spectroscopy were used to characterize the different modified electrodes. According to the characteristic that the immunocomplex formed by the specific binding of antigen and antibody reduced the effective diffusion cross-sectional area of the sensitive membrane, indirect detection of white horse diarrhea antigen by using potassium ferricyanide as molecular probe by cyclic voltammetry. Through the test to determine the criterion of yin and yang of chicken white dysentery, Δi> 2.5μA sentence as positive, Δi <2.5μA sentence as negative. The immunosensor has high specificity and reproducibility (RSD <6%) and high accuracy (coincidence rate of 88.9% with GB method). The immunosensor is placed in a dry state at 4 ℃ for 7 days and its response current is 90.2% of the initial current. Therefore, the immunosensor can be initially used for determination of yin and yang of chicken white dysentery and rapid screening.