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目的探讨黄芪卫矛合剂对糖尿病肾病大鼠的干预作用。方法用单侧肾切除并链脲佐菌素尾静脉注射建立大鼠糖尿病肾病模型,随机分为假手术组、糖尿病肾病组、糖尿病肾病+黄芪卫矛合剂组和糖尿病肾病+氯沙坦钾组。共持续12周,蛋白印迹法检测各组大鼠肾组织中肝细胞生长因子(HGF)和转化生长因子-β1(TGF-β1)蛋白表达。结果糖尿病肾病模型组HGF表达减少,TGF-β1蛋白表达增多,与假手术组比较均有非常显著性差异(P<0.01),两治疗组可增加HGF蛋白表达,降低TGF-β1蛋白表达,与模型组比较有显著性或非常显著性差异(P<0.01或P<0.05)。结论黄芪卫矛合剂能够通过增加糖尿病肾病大鼠肾组织中HGF蛋白表达,抑制TGF-β1蛋白表达,起到防治糖尿病肾病的作用。
Objective To investigate the intervention effect of Huangqi Weiyu Mixture on diabetic nephropathy rats. Methods A rat model of diabetic nephropathy was established by unilateral nephrectomy and streptozotocin intravenous injection. The rats were randomly divided into sham operation group, diabetic nephropathy group, diabetic nephropathy + Huangqi Weiyu Mixture group and diabetic nephropathy + losartan potassium group . For 12 weeks. Western blotting was used to detect the expression of hepatocyte growth factor (HGF) and transforming growth factor-β1 (TGF-β1) in the kidney of each group. Results Compared with the sham operation group, the expression of HGF and the expression of TGF-β1 in diabetic nephropathy model group were significantly decreased (P <0.01). The two treatment groups increased the expression of HGF protein and decreased the expression of TGF-β1 protein, The model group showed significant or very significant difference (P <0.01 or P <0.05). Conclusion Huangqi Weiyu Mixture can prevent and treat diabetic nephropathy by increasing the expression of HGF protein and inhibiting the expression of TGF-β1 in the kidney of diabetic nephropathy rats.