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我国沿海各省毛蚶养殖点广泛,但受生物性污染的情况不一。为了解毛蚶受甲肝病毒(HAV)污染程度,作者对浙江毛蚶主要养殖点内的33个组份毛蚶进行了检测。结果用ELISA法测到可疑阳性5份,在再次复测时均为阴性。用cDNA—RNA杂交法复核均呈阴性结果。实验表明,ELISA法简便、节约、快速、敏感,然特异性及重复性尚不及cDNA—RNA杂交法,但因其在基层便于实施,故在大量检测时,适于作为初筛广加应用。
There are a wide range of breeding sites in China’s coastal provinces, but they are subject to different levels of biological contamination. In order to understand the degree of hairy mildew caused by hepatitis A virus (HAV), the authors tested the 33 components of the catfish in the main breeding sites of Maoji in Zhejiang Province. Results Susceptible positive 5 were detected by ELISA, and negative again when retesting. Negative results were confirmed by cDNA-RNA hybridization. Experiments show that the ELISA method is simple, economical, rapid, sensitive, specificity and repeatability but not as good as cDNA-RNA hybridization method, but because of its implementation in the grassroots level, it is suitable for screening as a large number of applications in a large number of tests.