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目的:构建大鼠正畸牙根吸收模型,检测龈沟液中牙本质磷蛋白(DPP)的浓度变化规律。方法:雌性12周龄SD大鼠46只,其中16只取上颌第一磨牙及牙周组织,通过HE和TRAP染色观察不同时间点根吸收程度;另30只随机分为2组,持续加力28 d(CF组)和加力14 d后停止加力(IF组),收集龈沟液并通过ELISA检测DPP浓度。结果:持续加力14 d,DPP浓度逐渐升高,CF组与IF组间无显著差异(P>0.05);第14 d到28 d,CF组DPP浓度逐渐降低,而IF组DPP浓度在第21 d仍升高,组间有显著差异(P<0.01)。根吸收相对面积和DPP浓度显著相关(P<0.05)。结论:DPP作为牙的特异性蛋白,与正畸牙根吸收的修复相关,可能是早期诊断正畸牙根吸收的指标。
OBJECTIVE: To establish a model of orthodontic tooth root resorption in rats and detect the change of concentration of dentin phosphoprotein (DPP) in gingival crevicular fluid. Methods: Forty-six female Sprague-Dawley (SD) rats of 12 weeks of age were selected. Twenty-six of them received the maxillary first molars and periodontal tissues. HE and TRAP staining were used to observe the root resorption at different time points. The other 30 rats were randomly divided into 2 groups. After 28 d (CF group) and 14 d after application of force (IF group), GCF was collected and DPP concentration was measured by ELISA. Results: After 14 days of continuous application, DPP concentration gradually increased and there was no significant difference between CF group and IF group (P> 0.05). On the 14th to 28th day, the DPP concentration in CF group decreased gradually, 21 d still increased, there were significant differences between groups (P <0.01). The root resorption relative area and DPP concentration were significantly correlated (P <0.05). Conclusion: DPP, as a specific protein of the tooth, correlates with orthodontic root resorption and may be an indicator of early diagnosis of orthodontic root resorption.