论文部分内容阅读
拮抗链霉菌S24对黄曲霉、赭曲霉、黑曲霉等粮食和饲料中常见的曲霉菌具有广谱抗性,本试验通过牛津杯法测定抗菌物质的效价,研究了大孔吸附树脂对链霉菌S24产生的抗菌物质的吸附、解吸性能,筛选了解吸剂,并研究了抗菌物质的部分理化性质。结果表明,大孔吸附树脂AB-8对抗菌物质的吸附及解吸性能最好,其饱和吸附量为7.0822×104μg/g,最佳解吸剂为85%丙酮,以85%丙酮进行动态解吸,解吸率达93.82%。该抗菌物质对热稳定,对紫外线敏感,对有机溶剂不敏感,对酸敏感,对碱稳定,紫外全波长扫描发现该抗菌物质为多烯大环内酯类抗生素。
Antagonism Streptomyces S24 on Aspergillus flavus, Aspergillus octacin, Aspergillus niger and other food and feed Aspergillus versicolor broad-spectrum resistance test in this study by Oxford cup method to determine the titer of antibacterial substances, macroporous adsorption resin on Streptomyces S24 adsorption of antimicrobial substances, desorption properties, screening desorption agent, and studied some of the physical and chemical properties of antimicrobial substances. The results showed that macroporous resin AB-8 had the best adsorption and desorption properties for antibacterial substances with a saturated adsorption capacity of 7.0822 × 104μg / g and an optimal desorbent of 85% acetone. The desorption and desorption kinetics were carried out with 85% acetone. Rate of 93.82%. The antibacterial substance is stable to heat, sensitive to ultraviolet light, insensitive to organic solvent, sensitive to acid and stable to alkali. The antimicrobial substance is a polyene macrolide antibiotic by full-wave UV scanning.