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目的:研究蝎毒活性多肽对大鼠脑缺血-再灌注损伤的保护作用,并观察该保护作用对白细胞介素-1B(interleukin- 1B,IL-1B)蛋白表达的影响。方法:采用左侧大脑中动脉插入栓线(Middle cerebral artery occlusion,MCAO)方法制作大鼠脑缺血-再灌注模型,观察蝎毒活性多肽对脑缺血大鼠神经功能的影响;用免疫组化方法标记各组大鼠脑组织海马中IL-1B阳性细胞数量。结果:蝎毒活性多肽可降低大鼠局灶性脑缺血再灌注损伤行为学评分,改善因缺血所致神经行为功能缺失,且随着剂量的增加效应增强,并可以显著减少海马IL-1B阳性细胞的数量(P<0．05)。结论:蝎毒活性多肽对脑缺血损伤有一定的保护作用,且这种作用与其减少脑缺血早期IL-1B表达有关。
Objective: To study the protective effect of polypeptide from scorpion venom on cerebral ischemia-reperfusion injury in rats and observe its effect on the protein expression of interleukin-1B (IL-1B). Methods: The model of cerebral ischemia - reperfusion in rats was made by means of the left middle cerebral artery occlusion (MCAO). The effects of scorpion venom polypeptide on the neurological function of rats with cerebral ischemia were observed. Methods The number of IL-1B positive cells in the hippocampus of the rat brain tissue was marked. Results: Scorpion venom active peptides could decrease the behavioral score of focal cerebral ischemia-reperfusion injury in rats, improve the neurobehavioral deficits induced by ischemia, and increase the dose-dependent effect of scorpion venom active peptides, and significantly decrease the levels of IL- 1B positive cells (P <0.05). CONCLUSION: Scorpion venom peptide can protect cerebral ischemia injury and its effect is related to the decrease of IL-1B expression in early stage of cerebral ischemia.