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DNA条形码技术是利用基因组中一段短的标准序列进行物种鉴定的新技术。本研究对截获的一批非法入境木材样品的ITS1及TrnL-TrnF序列进行PCR扩增和测序。将测序结果与Gen Bank中的同源序列进行比对分析,发现未知样品与马来沉香(Aquilaria malaccensis)的序列相似性最高,达到99%~99.7%,遗传距离最小,为0.00~0.01。用邻接(NJ)法构建ITS1和TrnL-TrnF序列的系统进化树,未知样品均与马来沉香聚为一枝,bootstrap values分别为77%和100%。上述结果表明,未知样品为马来沉香,可利用ITS1和TrnL-TrnF从分子水平区分马来沉香与其他混伪种。
DNA barcoding is a new technique for species identification using a short set of standard sequences in the genome. In this study, ITS1 and TrnL-TrnF sequences from a batch of illegally entered timber samples were amplified by PCR and sequenced. The results of the sequencing analysis were compared with the homologous sequences of GenBank. The results showed that the similarity between the unknown sample and Aquilaria malaccensis was the highest (99% -99.7%) and the genetic distance was the smallest (0.00-0.01). The phylogenetic tree of ITS1 and TrnL-TrnF sequences was constructed by the neighbor-joining (NJ) method. The unknown samples were clustered together with the malachite, the bootstrap values were 77% and 100% respectively. The above results show that the unknown sample is Malay Incense, and ITS1 and TrnL-TrnF can be used to distinguish Malay radix and other pseudo-species from the molecular level.