本文应用聚合酶链反应（Ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ）方法对门诊患者中８６例女性宫颈分泌物和４２例男性尿道分泌物进行淋球菌检测，ＰＣＲ法检测敏感性为１００％，明显高于直接涂片（１１．７２％）和分离培养（１９．５３％）。因此ＰＣＲ法直接检测临床标本淋球菌具有特异、敏感、快速的优点，可以成为临床常规检查以取代直接涂片和分离培养，对于提高淋病的检出率和控制其传播具有非常重要的意义。 In this study, 86 cases of cervical secretions from women and 42 cases of male urethral secretions from outpatients were tested for gonorrhea by Polymerase Chain Reaction (PCR). The sensitivity of PCR was 100%, which was significantly higher than that of direct smear (11. 72%) and cultured in isolation (19.53%). Therefore, the direct detection of clinical specimens Neisseria gonorrhoeae by PCR method has the advantages of being specific, sensitive and rapid, and can be used as a routine clinical examination instead of direct smear and isolation for improving the detection rate and controlling the spread of gonorrhea.