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本研究以远杂9102×徐州68-4杂交后代衍生的重组自交系(RIL)的188个家系为材料,连续3年种植后检测其含油量及脂肪酸含量。结果表明,该RIL群体的含油量及脂肪酸变异丰富,从中获得了含油量稳定高于高值亲本的后代材料1份,油酸含量稳定高于高值亲本的材料23份。RIL群体的含油量、油酸和亚油酸含量以及油酸/亚油酸比的广义遗传力分别为0.849、0.761、0.874和0.887,表明这些性状的变异主要受基因型控制。利用前期构建的SSR遗传连锁图,结合3年主要品质性状鉴定数据,共检测到82个相关QTL,分布在11个连锁群上,其中与含油量、油酸、亚油酸和油酸/亚油酸比(油亚比)相关的QTL分别为15、21、21和25个,贡献率大于10%的主效QTL有23个,2年能重复检测到QTL有8个,3年重复检测到的有4个。其中,本研究新鉴定出的主效QTL有7个,重复性好的有5个,尤其是LG2上区间GM2839-GNB159,3年均定位到与油酸和油亚比相关的QTL,2年定位到与亚油酸相关的QTL,贡献率为5.80%~28.14%,该区间只有1.63c M。这些QTL的获得对于花生品质性状改良中亲本选配、后代标记辅助选择以及QTL精细定位具有重要意义。
In this study, 188 families of recombinant inbred line (RIL) derived from Yuanza 9102 × Xuzhou 68-4 were tested for their oil content and fatty acid content after three years of planting. The results showed that the RIL population was rich in oil content and fatty acid variation, and one of the progeny materials with stable oil content was higher than the high one. The content of oleic acid in the RIL population was higher than that of the high value parent material. The general heritability of oil content, oleic acid and linoleic acid content, and oleic acid / linoleic acid ratio of RIL population were 0.849, 0.761, 0.874 and 0.887 respectively, indicating that the variation of these traits was mainly controlled by genotypes. A total of 82 related QTLs were detected in 11 linkage groups based on the SSR genetic linkage map constructed in the previous stage and the identification data of the three major quality traits. Among them, the QTLs associated with oil content, oleic acid, linoleic acid and oleic acid / The number of QTLs with oleic acid ratio (oil to oil ratio) were 15, 21, 21 and 25, respectively, with 23 major traits contributing more than 10%, 8 repeated QTLs in 2 years and 3 repeated tests There are four. Among them, seven QTLs for main effect and 5 for reproducibility were newly identified in this study, especially the QTL for GM2839- Locating the QTL associated with linoleic acid, the contribution rate was 5.80% ~ 28.14%, the interval was only 1.63cM. The gain of these QTLs is of great importance to the selection of parents, marker-assisted selection and fine mapping of QTLs in the improvement of peanut quality traits.