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目的:研究在不同性别中,CYP4F2基因单核苷酸多态性与日本高血压的关系。方法:249例日本原发性高血压患者和207例年龄匹配的对照(日本人)分别分为整体组、男性组、女性组。选择CYPF2基因的5个SNPs(rs3093105、rs3093135、rs1558139、rs2108622、rs3093200),应用TaqManSNP基因分型的方法进行基因分型,分析CYP4F2基因单核苷酸多态性与高血压的相关性。结果:高血压患者和对照相比较,rs1558139基因型的显性模式分布(CCversusCT+TT)在整体组和男性组中显著不同(P=0.037,P=0.005),高血压患者的CC基因型频率显著高于对照的CC基因型频率。Logistic回归分析显示,在剔除高血压主要危险因素的干扰后,男性组rs1558139的CC基因型分布在高血压患者和对照之间仍然保持差异(P=0.026),而整体组高血压患者和对照之间CC基因型分布的差异消失(P=0.247)。对于男性,高血压组的C等位基因频率显著高于对照组(P=0.025)。结论:CYP4F2基因rs1558139的CC基因型和C等位基因可做为日本男性高血压的基因标记。
Objective: To study the relationship between CYP4F2 single nucleotide polymorphisms and Japanese hypertension in different genders. Methods: 249 Japanese patients with essential hypertension and 207 age-matched controls (Japanese) were divided into the whole group, the male group and the female group. Five SNPs (rs3093105, rs3093135, rs1558139, rs2108622, rs3093200) of CYPF2 gene were selected and genotyped by TaqMan SNP genotyping. The correlation between CYP4F2 single nucleotide polymorphisms and hypertension was analyzed. Results: Compared with the control group, the distribution of CC15 in the rs1558139 genotype was significantly different between the whole group and the male group (P = 0.037, P = 0.005). The frequency of CC genotype in hypertensive patients Significantly higher than the control CC genotype frequency. Logistic regression analysis showed that the distribution of CC genotype rs1558139 in male patients was still significantly different between hypertensive patients and controls (P = 0.026) after excluding the major risk factors for hypertension, whereas in the overall hypertensive patients and controls Differences in the distribution of CC genotypes disappeared (P = 0.247). For men, the C allele frequency was significantly higher in the hypertensive group than in the control group (P = 0.025). Conclusion: CC genotype and C allele of CYP4F2 gene rs1558139 can be used as genetic markers of Japanese male hypertension.