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植物名称:雪柑(甜橙)(Citrus sinensis)、椪柑(C.poonensis)、温州蜜柑(C.unshiu)、金弹(Fortunella crassifolia)。 材料类别:采用成熟果实,经灭菌处理后,摄取汁胞接种。 培养条件:基本培养基为MS,培养基蔗糖浓度为4％,琼脂0.7％,pH5.8～6.0。培养室温度为24±2℃,培养基中附加各种激素,组成不同激素水平的培养基进行培养试验。接种后暗培养,诱导愈伤组织,25天后继代黑暗培养,进行愈伤组织增殖。在分化培养基上,愈伤组织进行分化培养,每日光照12小时,光照强度1000～1200 lux。
Plant names: Citrus sinensis, C. poonensis, C. urshiu, Fortunella crassifolia. Material category: The use of mature fruits, after sterilization, the juice inoculated. Culture conditions: the basic medium for MS, sucrose concentration 4%, agar 0.7%, pH5.8 ~ 6.0. Incubation room temperature of 24 ± 2 ℃, additional various hormones in the medium, the composition of different hormone levels in culture medium test. After inoculation dark culture, callus induction, 25 days after subculture dark, callus proliferation. On the differentiation medium, the callus was differentiated and cultured. The daily light intensity was 12-12 hours and the light intensity was 1000-1200 lux.