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目的:建立小鼠磨牙区咀嚼刺激改变的实验模型,并观察小鼠在短期及长期内焦虑情绪的改变以及小鼠皮层和海马区单胺类神经递质的改变。方法:将2月龄C57BL/6雄性小鼠随机分成3组:对照组、磨牙磨除组(磨牙咀嚼刺激减少组)和磨牙拔除组(磨牙咀嚼刺激消除组),分别于处理后1周及2个月时进行旷场实验和高架十字迷宫实验,实验结束后处死小鼠,通过液相色谱-串联质谱法检测小鼠皮层和海马区单胺类神经递质的改变。结果:磨牙磨除组和磨牙拔除组小鼠在处理后1周及2个月内的自发活动能力都没有改变,并且1周内咀嚼刺激的改变不会对焦虑情绪产生影响,而2个月后咀嚼刺激的改变会增加小鼠的焦虑情绪。磨牙磨除组和磨牙拔除组小鼠海马区的多巴胺、肾上腺素和去甲肾上腺素的水平显著高于对照组,磨牙拔除组小鼠海马区的5-羟色胺水平显著高于对照组。结论:小鼠磨牙区长期咀嚼刺激的改变会增加小鼠的焦虑情绪并且会增加小鼠海马区单胺类神经递质的水平。
OBJECTIVE: To establish an experimental model of masticatory stimulation in mouse molars and to observe the changes of anxiety in mice in short and long term, as well as the changes of monoamine neurotransmitters in mouse cortex and hippocampus. Methods: Two-month-old C57BL / 6 male mice were randomly divided into three groups: control group, molars removal group (molars reduction group) and molars removal group (molars removal group) Open field test and elevated plus maze test were performed at 2 months. After the experiment, mice were sacrificed and the changes of monoamine neurotransmitters in cortex and hippocampus were detected by liquid chromatography-tandem mass spectrometry. Results: There was no change in spontaneous activity in the molar removal group and the molar removal group within 1 week and 2 months after treatment, and the changes in the chewing stimulation within one week did not affect the anxiety. However, in the 2 months Post-chewing stimulation changes will increase the anxiety in mice. The levels of dopamine, epinephrine and norepinephrine in the molar group and the molar group were significantly higher than those in the control group. The levels of serotonin in the hippocampus of the molar extraction group were significantly higher than those in the control group. CONCLUSION: Long-term chewing stimulation changes in mouse molar area increase the anxiety of mice and increase the levels of monoamine neurotransmitters in the hippocampus of mice.