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以抗白粉病的日本小麦品种Fukuho-komugi和以色列小麦Oligoculm杂交F1的DH(doubled haploid)群体107个系为材料,利用313个SSR标记和37个RFLP标记,对Fukuho-komugi和Oligoculm的白粉病成株抗性进行QTL分析。试验材料于2003—2004年度种植在北京2、003—2004和2004—2005年度种植在安阳,调查白粉病发病情况。构建了由350个位点组成的遗传连锁图,覆盖小麦21个连锁群,全长3 101 cM。采用复合区间作图法进行白粉病成株抗性QTL分析,在1A、2B4、B和7D上发现4个抗白粉病QTL,分别解释13.6%、6.6%、8.9%和12.7%的表型变异。抗白粉病基因及其紧密连锁分子标记的发掘,将为小麦抗白粉病育种的分子标记辅助选择提供理论和技术支持。
107 lines of doubled haploid (F1) population from Fukuho-komugi, a Japanese wheat cultivar resistant to powdery mildew, and F1 hybrids from Israeli Oligoculm were used as materials to screen powdery mildew of Fukuho-komugi and Oligoculm with 313 SSR markers and 37 RFLP markers Adult plant resistance QTL analysis. The test materials were planted in Beijing in 2003-2004 at 2,003-2004 and planted in Anyang in 2004-2005 to investigate the incidence of powdery mildew. A genetic linkage map consisting of 350 loci was constructed, covering 21 linkage groups of wheat with a total length of 3 101 cM. Four QTLs for resistance to powdery mildew were found on 1A, 2B4, B and 7D by composite interval mapping method, which explained the phenotypic variation of 13.6%, 6.6%, 8.9% and 12.7% . The discovery of powdery mildew resistant genes and their closely linked molecular markers will provide theoretical and technical support for the molecular marker-assisted selection of wheat powdery mildew resistance breeding.