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国内尚少见有胃液CEA、AFP联合测定对胃癌诊断价值的报道。为此,我们进行了探讨,现报告如下。 对象和方法 一、对象: (一)正常人:31例,选用本院健康体检的正常人,均无胃部疾患,心、肺正常,肝、肾功能试验正常。 (二)病人组:胃癌33例(全部病例均经Ⅹ线、胃镜、病理切片证实）,浅表性胃炎35例,急性胃炎30例。 二、方法: (一)胃液处理:参照有关文献进行。主要过程是:收集的胃液标本立即置-20℃低温冰箱,以后每隔1天复融一次,如此重复3次,于测定当天复融后称胃液离心沉淀,除去沉淀物,吸取上清液,用1mmol/L NaOH调正pH至6.5,然后用放免法测定。 (二)方法:放射免疫分析试剂盒由北方免疫试剂研究所提供,操作按说明书。
There is still a rare report on the diagnostic value of gastric juice CEA and AFP in the diagnosis of gastric cancer. To this end, we conducted a discussion and the report is as follows. Subjects and Methods I. Subjects: (1) Normal people: 31 patients were selected. Normal persons in our health examination did not have any stomach disorders, and the heart and lungs were normal. Liver and kidney function tests were normal. (b) Patient group: 33 cases of gastric cancer (all cases were confirmed by X-ray, gastroscopy, and pathological sections), 35 cases of superficial gastritis, and 30 cases of acute gastritis. Second, methods: (A) gastric juice treatment: refer to the relevant literature. The main process is: the collection of gastric juice samples immediately set -20 °C low temperature refrigerator, then re-melt every other day, so repeated three times, on the day after the determination of reconstituted gastric juice, said centrifugal sedimentation, remove sediment, absorb the supernatant, The pH was adjusted to 6.5 with 1 mmol/L NaOH and then determined by radioimmunoassay. (B) Methods: The radioimmunoassay kit was provided by the Northern Institute of Immunology, and instructions were followed.