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[目的]了解云南野马地区马铃薯致病疫霉对嘧菌酯和氟吡菌胺的抗性发生发展情况。[方法]采用菌丝生长速率法测定采自2012—2014年云南野马地区97株马铃薯致病疫霉对嘧菌酯和氟吡菌胺的敏感性,同时测定添加10mg/L水杨肟酸后病菌对嘧菌酯的敏感性。[结果]所有供试菌株在1 mg/L的嘧菌酯平板上均不生长,即对嘧菌酯均表现为敏感;2012、2013、2014年群体对氟吡菌胺的范围分别为0.279~0.884、0.252~0.582、0.171~0.710mg/L,其间无显著差异,EC_(50)总平均值为0.427mg/L。[结论]云南野马地区马铃薯致病疫霉尚未对嘧菌酯和氟吡菌胺产生抗性,97株菌株对氟吡菌胺的平均EC_(50)值可作为马铃薯致病疫霉对氟吡菌胺的敏感基线,为将来田间抗性监测提供参考。
[Objective] The research aimed to understand the occurrence and development of resistance to azoxystrobin and fluopicolide in Phytophthora infestans in the Mustang region of Yunnan. [Method] The mycelial growth rate method was used to determine the susceptibility of 97 potato pathogens to azoxystrobin and fluopicolide in the Mustang region of Yunnan from 2012 to 2014. The addition of 10 mg / L hydromoxime acid Susceptibility of bacteria to azoxystrobin. [Result] All the tested strains did not grow on 1 mg / L azoxystrobin plate, that is, all the tested strains were susceptible to azoxystrobin. The ranges of fluopyram in 2012, 2013 and 2014 were 0.279 ~ 0.884,0.252 ~ 0.582,0.171 ~ 0.710mg / L, there was no significant difference between them, the average value of EC 50 was 0.427mg / L. [Conclusion] Phytophthora infestans had no resistance to azoxystrobin and fluopicolide in Yunnan Mustang. The average value of EC50 of 97 isolates of fluopicolide could be used as a marker against Phytophthora infestans The sensitive baseline of the amikacin provides a reference for future field resistance monitoring.