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生物素是多种酶的必需辅助因子,其吸收需经多种转运步骤。本文研究生物素穿越入小肠细胞基侧膜泡(BLMV)的转运。用改良的自生Percall梯度法制备BLMV。转运研究采用快速滤过技术。BLMV制备物的纯度用酶学和功能标准确定。
Biotin is an essential cofactor for many enzymes and its absorption is subject to a number of transport steps. This article investigates the transport of biotin through the basolateral vesicle (BLMV) into the small intestine. BLMV was prepared by a modified self-generated Percall gradient method. Transport studies using rapid filtration technology. The purity of the BLMV preparation was determined using enzymatic and functional criteria.